ITGAM and Alzheimer disease: Microglia cells were stained using CD11b and CD45.2 antibodies and sorted by a FACSAriaIII; the number of microglia cells in the retina, identified as Cd11b + Cd45low cells (Fig. 3d), was comparable in the two genotypes (960 ± 150 cells in non-Tg, 1100 ± 100 in 3xTg-AD mice at 5–10 PNWs; 660 ± 100 non-Tg; 720 ± 150 3xTg-AD at 20–40 PNWs; n = 6 mice in each group, p = 0.1, two-way ANOVA) confirming immunofluorescence data (Fig. 2d).