BRAF and melanoma: Of note, treatment with necrostatin-1, a specific inhibitor of RIP1 kinase activity, did not affect apoptosis of Mel-CV and Mel-RM cells treated respectively with PLX4720 and AZD6244 (Fig. 1c), suggesting that RIP1 kinase activity is not involved in protection of melanoma cells from apoptosis induced by BRAF/MEK inhibitors.