Targeted sequencing of 16 known strictly CHD-related genes in 68 CHD patients identified variants previously linked to CHD including NKX2.5, ZFPM2, and MY6 as well as novel mutations in GATA4, NKX2.5, NOTCH1, and TBX1 [39]. The gene discussed is NKX2-5; the disease is coronary artery disorder.