The TGF-β/Smad2/3 pathway, which is closely related to myocardial fibrosis, was also impaired by 1-DNJ administration, and this result verified the results from LCA lectin affinity histochemical analysis, as well as lectin blot analysis of the α-linked fucosylation of the N-glycoproteins expressed in the left ventricular myocardium. Here, SMAD2 is linked to Myocardial fibrosis.