To directly evaluate whether EcoHIV reservoirs in T cells in vivo can be induced by epigenetic modulators analogously to those in HIV-infected persons [63, 65], 6 weeks after infection mice were treated with different combinations of activating antibodies and epigenetic inducers for 2 days and then induction of vRNA in splenic CD4+ T cells was evaluated (Fig 3F, left panel). This evidence concerns the gene CD4 and infection.