Comparing normal donors and HCC patients, our TLN biochip using EV AFP and GPC-3 mRNAs provided an AUC (area under the ROC curve) of 0.995, better than that of a single marker (0.936 for AFP protein, 0.947 for EV AFP mRNA, and 0.979 for EV GPC3 mRNA). Here, GPC3 is linked to hepatocellular carcinoma.