Furthermore, to investigate which stimuli were mainly responsible for mTORC2 activation in TFH cells, we stimulated splenocytes from wild-type C57BL/6J mice at day 8 of infection with different combinations, including anti-CD3 plus anti-CD28, anti-CD3 plus anti-ICOS, anti-CD3 only, anti-ICOS only, or CXCL13 only. The gene discussed is CD28; the disease is infection.