While the pro-senescence effect of SHK (10 mg/kg) led to strong tumor inhibition in vivo (55.84% in A549 xenograft mice, and 50.98% in H1299 xenograft mice, respectively), meanwhile, cell cycle, EdU, and apoptosis assays also revealed that SHK notably arrested cell proliferation and activated cellular apoptosis simultaneously. The gene discussed is SHPK; the disease is neoplasm.