Consistently, lung cancer H2228 cells with acquired resistance to the ALK inhibitor NVP-TAE684 also showed increased Hedgehog pathway activation (seen as both a significant increase in ciliary localization of SMO following receptor engagement [Figures 3E and 3F], and an increase in the levels of GLI1 ([Figure 3G]) compared to parental cells. This evidence concerns the gene ALK and lung cancer.