POLI and colorectal neoplasm: To investigate the clinical significance of our results with regards to this activity, and to determine whether there is a link between PolI complex disruption and NF-κB pathway activation in a whole tissue setting, we treated biopsies of fresh, surgically resected human colorectal tumours with pharmacological doses (0–100 μM, 1 h) of aspirin ex vivo (Figure 9A).