Many investigators have argued EpCAM is a highly immunogenic tumor-associated antigen.17 It has been shown that EpCAM over-expression is required for breast cancer cell lines to proliferate, migrate, and become invasive.12, 17 Although this statement still holds true, the results of this study demonstrate that the amount of EpCAM expression per breast cancer cell line can differ greatly. This evidence concerns the gene EPCAM and neoplasm.