An upregulation of transcript for cathepsins, including Cathepsin L (Ctsl) and Legumain (Lgmn) was seen in spleens 1–5 days after experimental stroke in comparison to sham-operated controls (Figure 4I) and increased expression of Ctsl mRNA was confirmed by RT-qPCR suggesting there was no impairment in proteolytic processing of endocytosed material in the spleen after experimental stroke (Figure 4J). Here, LGMN is linked to stroke disorder.