Except for the abovementioned factors, there were no significant differences in almost all factors, including normalization of CEA values post NAT, RECIST evaluation, pathological grade, distance from anal verge after NAT, circumferential resection margin (CRM), lymphovascular invasion, improved invasion (pT), and lymph node metastasis (pN), pLPLN metastasis, and ypStage. The gene discussed is CEACAM5; the disease is metastatic malignant neoplasm in the lymph nodes.