In vitro stimulation of TLR2/6 by T. cruzi GPI-mucins leads to the production of pro-inflammatory cytokines such as IL-12 and TNF, as well as NO, which are related to a Th1-focused immune response that is important to control parasitemia and tissue parasitism (Cardoso et al., 2016). This evidence concerns the gene TLR2 and parasitic infectious disease.