In this context, we propose the spheroid 3D culture system to evaluate anti-tumor drug-induced immune response; in particular, we analyzed the anti-tumor effects of Vδ2 T lymphocytes triggered by Zol and/or the anti-epidermal growth factor receptor (EGFR) humanized antibody (huAb) cetuximab (Cet) on different CRC cell lines. The gene discussed is EGFR; the disease is neoplasm.