Even though it was not found to be expressed in noncancerous tissues other than tissues of the testis and the endometrium, PRAME was missed in the stringent in silico screen because in the 54 breast cancer specimens analyzed, PRAME expression was restricted to only 11 (20%) samples of which only 2 (4%) demonstrated appreciable expression levels (data not shown). The gene discussed is PRAME; the disease is breast carcinoma.