The results shows that ANIT induced cholestasis is associated with down-regulation of UGT1A1, MRP2, BSEP, OCT1, NTCP, MDR1, OATP1A1, OATP1A2, and OATP1A4 and YCHD can up-regulate UGT1A1, MRP2, BSEP, OATP1A2, OATP1A4, OCT1, and NTCP during the ameliorative effect of cholestasis. This evidence concerns the gene SLC22A1 and cholestasis.