Analysis of OPTN puncta (Figure 4D) exhibited increased mean puncta per cell across all PD model stages, but gradually decreasing mean puncta per cell with stage; 25 ± 1.1, 56 ± 3.0, 44 ± 1.7, and 42 ± 1.5 (p < 0.001 for all PD model stages) for control, 24 h, 5 d, and end-stage, respectively. The gene discussed is OPTN; the disease is Parkinson disease.