ANXA5 and cancer: To find out whether depletion of KIAA0100 could compromise cancer cells, anoikis resistance potential and result in cell death in suspension, anoikis was detected by Annexin V/PI (Propidium Iodide) staining 48 h after the treatment of KIAA0100 siRNA, with the result corresponding to the time-point showing maximal protein depletion.