Moreover, the identified KIAA0100 interaction with the microtubule network and HSPA1A was in turn confirmed to be co-localized in the breast cancer cell line MDA-MB-231, suggesting the potential molecular mechanism of KIAA0100 in the breast cancer cell line MDA-MB-231 was consistent with the discovery from the HEK293 model cells in our study. The gene discussed is HSPA1A; the disease is breast cancer.