HSPA1A and breast carcinoma: In contrast, treating cells with varying concentration of Demecolcine (0.5, 5, 50 μM) did not show a similar effect of losing cell aggregation and viability (Figure 10A,B) on the cells, indicating depolymerization of microtubules alone in the suspended MDA-MB-231 cells was not enough to introduce anoikis, suggesting that the association of KIAA0100 with HSPA1A but not microtubule might be more likely responsible for the anoikis resistance in the breast cancer cell line MDA-MB-231.