In the case of colon cancer cells, conditioned medium from primary tumour cells enhanced phosphorylation of Cx43 and GJ formation between tumour and endothelial cells via the molecular chaperone heat shock protein 27 (HSP27), while metastatic colon cancer cells induced expression of Cx32 through action of the chemokine receptor CXCR2 [125]. Here, GJA1 is linked to colonic neoplasm.