To identify tumor‐suppressive miRNAs repressed by DNA hypermethylation in highly tumorigenic GC, we isolated EpCAM+/CD44+ GC cells from primary GC tumor tissues and performed methyl‐CpG‐binding domain (MBD) sequencing (MBD‐seq) and miRNA sequencing (miRNA‐seq). This evidence concerns the gene CD44 and gastric cancer.