Recently, several targets of miR‐1271 (ZEB1, TWIST1, CCNG1, FOXQ1, ALK, HOXA5, GPC3, IGF1R, IRS1, mTOR, and BCL2) were identified in various cancers using a knowledge‐based approach.24, 25, 26, 27, 42, 43 However, since genome‐wide screening has not yet been done, we performed RNA‐seq analysis to identify miR‐1271 target genes, and GSEA reflected the functional characteristics of the final 40 miR‐1271 targets. This evidence concerns the gene GPC3 and cancer.