To identify the relationship between over expressed WT1 and NSCLC (non-small cell lung cancer) development as well as to reveal the antitumor activity of WT1-targeted shRNA in vivo, NSCLC model was generated by subcutaneously injecting 5 × 106 cells into Balb/C nu/nu mice. This evidence concerns the gene WT1 and non-small cell lung carcinoma.