RHOA and hypertensive disorder: Although our findings conflict with a previous study in GPR35 knockout mice, existing evidence of pathological roles for Gα13/RhoA/ROCK signaling in the cardiovascular system, including prohypertensive and prohypertrophic effects, provide a rationale for why GPR35 knockout mice are protected from cardiovascular disease.26–28 More in-depth investigation of the activation of these components in GPR35 knockout models of cardiovascular disease may yield insights into potential mechanisms of Ang II–induced hypertension resistance in GPR35 knockout mice.