In this study, though the Slit3 shCTL cells showed baseline chemoresistance to all the chemotherapeutic agents, we showed that Slit3 repression, which was observed in around 50% of HCC patients of this study, was one of the contributing factors towards the development of resistance to a broad range of chemotherapeutic agents. This evidence concerns the gene SLIT3 and hepatocellular carcinoma.