Although, no significant difference was seen in Slit3 gene expression between HCC and adjacent non-tumorous livers, we observed that 42.5% (N = 17) of the HCC patients showed Slit3 down-regulation (tumor/non-tumor< 1), suggesting that Slit3 repression was a frequent event associated with HCC. Here, SLIT3 is linked to hepatocellular carcinoma.