Within HCC, recombinant Slit3 treatment may benefit at two levels: Firstly, Slit3 can repress the growth of HCC tumor or perhaps even cause a shrinkage of the established tumor; Secondly, Slit3 could be applied as an adjuvant therapy which enhances the effectiveness of other chemotherapeutic agents such as sorafenib, oxaliplatin and 5-FU, as shown in our stable cell-line models. This evidence concerns the gene SLIT3 and hepatocellular carcinoma.