In this study, we aimed to make a step-wise approach: (1) identifying meningioma-specific candidate biomarkers (EMA, PDGF-β, VEGF-α and SSTR-2); (2) selecting the most promising tumour-specific marker; and (3) confirming its expression in in vitro cultures derived from fresh meningioma specimens. This evidence concerns the gene MUC1 and meningioma.