To examine whether G-CSF reduced delayed (i.e., 6 h after stroke) tPA-induced HT and cerebral infarction in a TE model, we evaluated hemorrhage volume and extent of cerebral infarction 3 days after stroke using spectrophotometric hemoglobin assay and 2,3,5-triphenyltetrazolium chloride (TTC) staining, respectively. This evidence concerns the gene CSF3 and stroke disorder.