The intratumoral administration of oncolytic adenovirus co-expressing IL-23 and p35, the subunit of IL-12, stimulated an antitumor effect in a murine B16-F10 syngeneic tumor model, by inducing the up-regulation of IL-12, IL-23, IFN-γ, and TNF-α within the tumor tissues and reducing the Tregs frequency [73]. This evidence concerns the gene TNF and neoplasm.