Western blotting analysis showed that purified antihuman CT83 mAb 7G4 could specifically recognize the recombinant CT83 protein and the endogenous CT83 protein of the RKO, SW1116, NCI‐H1299, HuH‐7, HeLa, MCF‐7, MDA‐MB‐231, HNE‐1, CNE‐2 cancer cell lines with molecular weight of about 14 kDa, which was corresponding to the molecular weight of recombinant CT83 protein (Figure 3A). Here, CT83 is linked to cancer.