In detail, pHRE-Egr1-HSV-TK was transfected into hepatoma cells by using PEI-Mn0.5Zn0.5Fe2O4 nanoparticles (PEI-MZF-NPs) as the gene transfer vector, and subsequently 131I-antiAFP McAb-GCV-BSA-NPs were intervened into hepatoma, and then the tumors were directionally heated in an alternating magnetic field by adopting PEI-MZF-NPs as magnetic media. Here, EGR1 is linked to hepatocellular carcinoma.