On the basis of our previous studies, the present work was undertaken to investigate FSH receptor binding inhibitor-8 (FRBI) effects upon the related factors (including K-Ras, c-Myc and VEGF, FSHR) to ovarian cancer, to evaluate the effect of FRBI on mRNA and protein levels of FSHR and ERβ in COCs, also to determine changes of protein kinase A (PKA) in sheep granulosa cells, further to elucidate signaling pathway of FRBI effects. Here, VEGFA is linked to ovarian cancer.