MSLN and familial pancreatic carcinoma: In an initial in vitro screen to identify immune recognition hotspots within the mesothelin molecule (pertaining to T-cell reactivity), the mesothelin601-615 peptide (MQEALSGTPCLLGPG) which is part of the mature mesothelin molecule expressed on the surface of pancreatic cancer cells, was identified as an immunodominant mesothelin peptide (Supplementary Figure 1 and Supplementary Table 2).