The results showed that ISL protected pancreatic injury in AP mice in a dose-dependent manner and was positively correlated with the degree of Nrf2/HO-1 activation, suggesting that ISL may protect AP through activation of Nrf2/HO-1, which was confirmed by two inhibitors of the Nrf2/HO-1 pathway (ML385 and Znpp). This evidence concerns the gene HMOX1 and alkaline phosphatase measurement.