So, the aims of this study are to investigate macrophage changes and the HO-1 positive M2 macrophage expression after being treated with EA and to further explore whether the antioxidative stress and anti-inflammatory effects mediated by the HO-1 positive M2 macrophages participate in the protective effects of EA on ICC in diabetic mice. The gene discussed is HMOX1; the disease is intrahepatic cholangiocarcinoma.