Treatment with CO significantly decreased the TNF-α expression (NAFLD + CO = 3.87 ± 0.70 positive cells/fields) (Figure 1(c)) but greatly increased TGF-β expression (NAFLD + CO = 14.17 ± 0.83 positive cells/fields) (Figure 1(d)). This evidence concerns the gene TGFB1 and metabolic dysfunction-associated steatotic liver disease.