NPY and obesity due to melanocortin 4 receptor deficiency: Obesity rats have increased methylation of the Tsc1 gene promoter and expression of mTORC1, AgRP, and NPY gene and decreased PoMC in the hypothalamus; EA treatment can lower body weight with the extent of body weight decrease being 11.47% and body fat and the body fat rate in DIO rats by demethylation of the Tsc1 gene promoter, decreasing expression of mTORC1, AgRP, and NPY and increasing PoMC.