GLI1 and colorectal carcinoma: To assess the preclinical utility of itraconazole for the treatment of patients with CRC, nonobese diabetic severe combined immunodeficient gamma (NSG) mice were engrafted with the Gli-negative/Hh pathway–inactive but itraconazole-responsive HT55 CRC cell line and were dosed with oral itraconazole or control (Fig. S4 D).