However, as a whole, the questionnaires were likely to capture previous Zika-associated symptoms since: (i) of 23 cases that were ZIKV-positive at enrollment, 6 (rRT-PCR-negative IgM-positive) did report Zika-related symptoms; (ii) symptoms such as arthralgia may be persistent and re-occurring [32]; (iii) the participants were asked to report previously diagnosed ZIKV infections, likely capturing previous Zika-associated symptoms; and (iv) the 62% of asymptomatic infections are well within the previously reported range. This evidence concerns the gene CD40LG and infection.