BAX and type 1 diabetes mellitus: Figure 5(a) shows that mitochondrial Bax : Bcl-xL ratio was significantly increased in C + DEN, T1DM, and T1DM + DEN relative to C, indicating that, in both DEN treatment and diabetic state, the liver is promoted to a proapoptotic state. Besides, cytochrome c cytosolic release shows significant differences in T1DM + DEN with respect to C + DEN and T1DM groups demonstrating slight activation of the apoptotic pathway (Figure 5(b)).