GAPDH and B-cell chronic lymphocytic leukemia: Using total RNA isolated from the peripheral blood of healthy individuals (n=3) and chronic lymphoid leukemia (CLL) patients (n=3), cDNA reverse transcription was performed followed by a real-time PCR using the designed primers: no PCR product was detected for the region containing the SNP, although a control using primers for the GAPDH gene was successful (data not shown).