The studies of chemo-immunotherapy on animal models of colon cancer showed that treatment effectiveness did not correlate with the degree of tumor cell apoptosis induced by anthracyclines or other drugs but with the potential of the drugs to induce rapid mobilization of intracellular chaperone calreticulin (CRT) to the surface of tumor cells before initiation of apoptosis. This evidence concerns the gene CALR and malignant colon neoplasm.