EGFR and cancer: Using optimized lentivirus transfection condition, we conducted CRISPR/Cas9 gene-editing strategy to disrupt EGFR gene sequence and eliminate EGFR protein expression in both MDA-MB-231 cell and SW579 cells, ensuring that the high and specific gene-edit efficiency from our CRISPR/Cas9 system can be easily applied on any human cancer cells.