In addition, the expression levels of occludin and claudin-4 in the pretreated + treated-Bifico-colitis group were slightly higher than those in the pretreated-Bifico-colitis group with no significant difference (P = 0.246 for occludin and P = 0.345 for claudin-4) (Figure 4(b)). Here, CLDN4 is linked to colitis.