TYR and melanoma: In the present study, the murine B16F10 melanoma cellswere used because they possess melanogenic mechanism, are known to haveintracellular tyrosinase, and can generate melanin, which are related to α-MSHstimulation and melanogenesis (37).Tyrosinase activity, melanin content, and cell viability were the invitro assays used to screen antimelanogenesis in present study.