Serum and tissue levels of TNF-αin all four groups after receiving their respectivetreatment, are shown in Figure 6A, B. Serum TNF-αlevel in rats fed with HF/HC diet was higher than thatof rats fed with the normal diet on days 0 (2.35 fold),14 (2.92 fold) and 28 (3.7 fold) (P<0.05, Fig .6A).Cardiac TNF-α level was also higher in diabeticrats compared to control rats on different days afterdiabetes induction (P<0.05, Fig .6B). The gene discussed is TNF; the disease is hydrops fetalis.