To functionally examinethe effect of the dominant variant we had identified, wedownregulated LRSAM1 in neuroblastoma SH-SY5Ycells and overexpressed the ancestral and the c.20471G>A mutant LRSAM1 in LRSAM1 knocked down cells.We hereby report the effects of LRSAM1 downregulationon SH-SY5Y cells and the inability of mutant LRSAM1to reverse the phenotype, contrary to the ancestral form. The gene discussed is LRSAM1; the disease is neuroblastoma.