We studied a retrospective cohort of 160 consecutive surgically treated NSCLC patients with available frozen tumor samples for expression of EMT markers (CDH1, CTNNB1, CDH2, and VIMENTIN), inducers (TGFB1, c‐MET, and CAIX), and transcription factors (EMT‐TF:SNAI1, SNAI2, ZEB1, TWIST1, and TWIST2). The gene discussed is TWIST1; the disease is neoplasm.