P. cathemerium lineage Pcat6 was detected in salivary glands of all species except Cx. pipiens, whereas lineage Pcat8 was detected in only Cx. stigmatosoma and Cx. tarsalis. The probability of sporozoite infection by P. cathemerium parasites (based on salivary gland infections by PCR) differed significantly among mosquito species based on the logistic regression with adjustment for the trend over time post-infection (Fig. 3). The gene discussed is PCAT6; the disease is infection.