In this study, animals were injected with intraperitoneal dose of 55 mg kg−1 of STZ followed by an intraperitoneal dose of 210 mg kg−1 of nicotinamide, which prevented STZ from producing a complete destruction of pancreatic β-cells in order to create T2D animal model of relative insulin secretion (Furman, 2015). Here, INS is linked to type 2 diabetes mellitus.