LGR5 and neoplasm: On a similar theme, Su et al used methylation-specific PCR to analyse the status of the LGR5 promoter in six CRC cell lines and 169 primary CRC samples.53LGR5 promoter methylation was completely absent in normal colonic tissue; however, varying degrees of methylation were observed in half of the CRC cell lines examined (HCT116, complete methylation; SW480 and SW620, partial methylation) and 40% of the primary tumour samples, where it correlated with higher tumour grade.