MAP2K1 and neuroblastoma: Stable knockdown of LGR5 in APC mutant LoVo cells led to disorganisation of the cytoskeletal structure and decreased cell adhesion, through disruption of E-cadherin/β-catenin interaction.75 Outside of CRC, LGR5 expression can impact upon MAPK signalling, which affects the survival of neuroblastoma cells.76 LGR5−targeted siRNA treatment of neuroblastoma cell lines resulted in a dramatic reduction of phosphorylated MEK1/2 and ERK1/2, with an increase in pro-apoptotic BimEL.