Further support for a tumour limiting role was provided by the Wu study which reported that LGR5 overexpression alone (or in combination with RSPO2) was sufficient to inhibit HT29 cell proliferation.57 This result would appear to be at odds with the study mentioned previously, where LGR5 overexpression promoted HT29 cell proliferation.27 This difference may have arisen through the contrasting LGR5 overexpression systems used between the studies, with the Hsu study employing transient LGR5 overexpression, and the Wu study adopting stable LGR5 overexpression. Here, RSPO2 is linked to neoplasm.