A more recent study by Wu and colleagues identified RSPO2 as downregulated in human CRC cell lines and primary tumours, relative to normal colonic mucosa, due to promoter hypermethylation.57 RSPO2 overexpression had an inhibitory effect on CRC cell line growth and Wnt signalling output, whilst increasing LGR5 expression. Here, RSPO2 is linked to neoplasm.