LGR5 and colorectal carcinoma: In support of this, silencing LGR5 through both shRNA and siRNA approaches in LIM1899 and LIM1215 CRC cell lines increased invasion, anchorage-independent growth, and enhanced tumourigenicity in xenograft experiments.56 Conversely, overexpression of LGR5 resulted in augmented cell adhesion, reduced clonogenicity, and attenuated tumourigenicity.