To determine whether DSGOST promotes EMT in gastric cancer cells, the exposure of these cells to DSGOST was studied in a time-dependent manner, and EMT markers, such as E-cadherin, N-cadherin, β-catenin, vimentin, Slug, Snail, p-Smad2, and p-Smad3, were examined. Here, SMAD3 is linked to gastric cancer.